Journal: medRxiv
Article Title: Intracalvariosseous bone marrow injection detours the blood-brain barrier as a novel drug delivery approach for pre-clinic and clinic trials in stroke
doi: 10.1101/2024.03.03.24303548
Figure Lengend Snippet: a. Process of injecting Cy3-NA1 by ICO, 647-CD31 by intravenous and 488-Lyve1 by intracisternal magna. b. Skull-dura-brain integrated tissue processing timeline. c. Overall scan results of lymphatic vessels, blood vessels and Cy3-NA1, scale=2 mm. d. Cy3-NA1 enters brain tissue through microchannels (white arrows) between the skull and brain parenchyma after ICO injection, scale=1 mm, inset=300 μm.
Article Snippet: After injecting 5 ul of Cy3-labeled NA1 (Cy3-NA1) into the mice’s skull bone marrow, 10 ul of 488-labeled Rabbit polyclonal Lyve1 antibody (R&D, FAB2125G) was immediately injected through the cisterna magna at a speed of 2 ul/min.
Techniques: Injection